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KMID : 0366119760040040145
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Korean Journal of Applied Microbiology & Bioengineering
1976 Volume.4 No. 4 p.145 ~ p.151
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Studies on the Microbial Glucose Isomerase
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Chung Tai-Wha
Han Moon-H.
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Abstract
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Cultural characteristics of a strain of Streptomyces sp. K-14 (KFCC 35051) producing glucose isomerase were demonstrated. The glucose isomerase was produced when the strain was grown in the medium containing pure xylan or xylan-containing materials such as wheat bran or corn cob. The optimum condition was attained in a culture medium composed of 3% wheat bran or corn cob, 2% corn steep liquor, 0.1 % MgSO₄. 7H₂O and 0.012 % CoSO₄·7H₂O for the production of the glucose isomerase. The production of the enzyme reached to a maximum level when the strain was cultured for 40 hrs 30℃ and pH 7.0.
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